Distribution of parasite loads in blood and serum between chronic patients with distinct clinical manifestations of Chagas disease. A. Black symbols represent parasite loads quantified in blood samples by multiplex Real Time qPCR. B. Gray symbols represent the respective parasite loads in serum samples. Median values are indicated by a straight horizontal line. MF = Mixed Form (n = 7); CF = Cardiac Form (n = 23); DF = Digestive Form (n = 4); IF = Indeterminate Form (n = 6).