Expression profiling of regulated miRNAs by Real time PCR. (A) miR-989, (B) miR-219, (C) miR-277, (D) miR-285, (E) miR-34, (F) miR-1174, (G) miR-9a and (H) miR-210 were profiled in larva male (LM), larva female (LF), pupa male (PM), pupa female (PF), adult male (AM) and adult female (AF) mosquito. Y axis depicts fold change in miRNA expression in samples compared with adult female mosquito (AF), fold change in which was taken as 1. (I) Fold change in miRNA-989 expression in ovaries from scrambled (SCR) and antagomir (ANTIMIR) injected mosquitoes. Y axis depicts fold change in miRNA expression in samples compared with PBS injected ovaries, fold change in which was taken as 1. 5.8 s RNA was taken as an endogenous control.