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Table 4 List of enriched pathways functional during different stages of mosquito development

From: Dynamic expression of miRNAs across immature and adult stages of the malaria mosquito Anopheles stephensi

(I) miRNAs differentially expressed in male and female adult mosquitoes
  Down-regulated miRNAs in females: miR-2c, miR-285, miR-219, miR-7 and miR-2765
  Up-regulated miRNAs in females: miR-989
  Significant clusters identified Enrichment score
1 Cellular polysaccharide biosynthetic process 2.1
2 Chaperone 1.8
3 Hexose metabolic pathway 1.3
4 Heme binding 1.1
5 Phagocytotic pathways 1.09
(II) miRNAs regulated from larval to pupa transition of male mosquito
  Down-regulated miRNAs in pupa male: miR-2c, miR-375, miR-7, miR-1175, miR-263a, miR-1174 and miR-34  
  Up-regulated miRNAs in pupa male: miR-100, miR-193, miR-1-3p, miR-1890, miR-277, miR-989 and miR-285  
  Significant clusters identified Enrichment score
1 phagocytosis and membrane organization 2.6
2 polysaccharide metabolic process 2.1
3 protease activity 1.9
4 peptidase activity 1.6
5 nuclear transport 1.1
(III) miRNAs regulated from transition of pupa to adult male mosquito
  Down-regulated miRNAs in adult male: miR-2765, miR-193, miR-282, miR-989, miR-2a-5p, miR-9a, miR-1890 and miR-965  
  Up-regulated miRNAs in adult male: miR-10-5p, miR-219, miR-2c, miR-210-3p, miR-190-3p, miR-34 and miR-1891  
  Significant clusters identified Enrichment score
1 gelsolin –actin binding protein 2.2
2 polysaccharide biosynthetic process 2
3 zinc finger 1.8
4 vesicle mediated transport 1.7
5 protein biosynthesis 1.6
6 translation 1.5
7 vitamin and cofactor binding 1.4
8 nucleoside binding 1.3
9 protein catabolic process 1.2
10 insect pheromone/odorant binding protein phBP 1.1
(IV) miRNAs regulated from larval to pupa transition of female mosquito
  Down-regulated miRNAs in pupa female: miR-7, miR-263a, miR-375, miR-317, miR-34, miR-1174, miR-315 and miR-993-3p  
  Up-regulated miRNAs in pupa female : miR-1-3p, miR-let-7, miR-277, miR-133-3p, miR-285, miR-193, miR-998 and miR-184b  
  Significant clusters identified Enrichment score
1 endocytosis 3
2 polysaccharide metabolic process 1.9
3 cell-redox homeostasis 1.7
4 peptidase 1.5
5 amino acid metabolic pathway 1.4
(v) miRNAs regulated from transition of pupa to adult female mosquito
  Down-regulated miRNAs in adult female: miR-2765, miR-2c, miR-1-5p, miR-193, miR-282, miR-2944a-5p, miR-285 and miR-9a  
  Up-regulated miRNAs in adult female : miR-927-5p, miR-375, miR-980, miR-989, miR-210-3p, miR-317, miR-34 and miR-1891  
  Significant clusters identified Enrichment score
1 phagocytosis 2.4
2 cellular carbohydrate biosynthetic process 2.1
3 protein biosynthesis 1.6
4 vitamin binding 1.5
5 golgi membrane 1.1
6 ATP binding 1.1
7 hydrolase activity 1.1
8 transcription 1.1
  1. These pathways were identified using NIH DAVID functional annotation clustering tool with E score =0.1.