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Table 5 Genotyping of Toxoplasma gondii DNA isolated from heart tissue from livestock animals in St. Kitts and Nevis

From: Toxoplasma gondii in livestock in St. Kitts and Nevis, West Indies

   PCR-RFLP marker  
Reference/Sample Species 5′SAG2 3′SAG2 SAG3 BTUB GRA6 Genotype
B1 – Type I   I or II I or III I I I I
M4 - Type II   I or II II II II II II
NED - Type III   III I or III III III III III
6 Pig Na Na III Na Na IIIa
9 Pig I or II I or III I I I I
10 Pig Na Na III III III III
23 Pig III I or III III Na III III
29 Pig III Na Na Na Na IIIb
36 Sheep I or II II II Na I Re-assort. I and IId
37 Sheep Na Na II Na I Re-assort. I and IId
69 Pig Na Na Na I Na Ic
72 Pig III I or III III Na Na III
83 Sheep III Na Na Na Na IIIb
91 Pig I or II II III II and III II Mixed II and III
97 Pig I or II II III Na Na Re-assort. II and IIId
98 Pig Na Na III Na III III
99 Pig III I or III III III III III
123 Pig III I or III III III III III
136 Goat III I or III III Na Na III
158 Sheep III I or III III Na Na III
195 Goat I or II Na Na Na Na I or IIb
203 Goat I or II Na Na Na Na I or IIb
249 Pig I or II Na Na Na Na I or IIb
  1. Na = no amplification.
  2. aBased on a single allele at the SAG3 locus.
  3. bBased on a single allele at the 5′SAG2 locus.
  4. cBased on a single allele at the GRA6 locus.
  5. dPossible re-assorted markers.