Figure 3

Phylogenetic relationships of Ctenocephalides felis and Ctenocephalides canis based on nucleotide sequence of the mtDNA cox 1. The tree was inferred using the Minimum Evolution method with distances calculated using Kimura 2-parameter method. There were a total of 658 positions in the final dataset. For the tree shown, all ambiguous positions were removed for each sequence pair. The numbers above the branches indicate percentage of 1000 replicate trees in which the associated taxa clustered together in the bootstrap test. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The tree was rooted using Bradiopsylla echidnae mtDNA cox1 sequence (not shown). The scale is in the units of the number of base substitutions per site. Evolutionary analyses were conducted in MEGA6. Flea species is shown on the right and terminal nodes are labelled with their unique identifier and country of origin. Fleas from India are in black boxes followed by the locality where it was collected, a map is shown in the inset.