Up-regulated IL-17A response in MF
individuals. Isolated PBMCs (2 x 105/well) from NEN (n = 12) or O. volvulus-infected MF+ (n = 164) or a-MF (n = 46) patients were stimulated with MSP-1 (0.25 μg/ml) for 72 hours. Thereafter, levels of IFN-γ (A) and IL-17A (B) were measured in the culture supernatants via ELISA. Data are plotted as fold increase over unstimulated controls. Graphs show box whiskers with median, interquartile ranges and outliers. Statistical significances between the indicated groups were obtained after Kruskal-Wallis and Mann–Whitney tests. (C) MSP-1 induced IFN-γ levels were correlated with the amount of ECP in plasma  and (D) MSP-1 induced IL-17 responses were correlated with the percentage of neutrophils . Both correlations were tested for statistical significance using spearman correlation test.