Figure 1From: Apoptosis mediated leishmanicidal activity of Azadirachta indica bioactive fractions is accompanied by Th1 immunostimulatory potential and therapeutic cure in vivo Antileishmanial effect of A. indica fractions on L. donovani promastigotes. (a). Growth Kinetics Assay. 2 × 106 ml−1 stationary phase promastigotes were grown in the presence of different A. indica fractions: A. indica leaves hexane (ALH), ethanol (ALE) and aqueous (ALA); A. indica bark hexane (ABH), ethanol (ABE) and aqueous (ABA); A. indica seeds hexane (ASH), ethanol (ASE) and aqueous (ASA). Additional experimental groups included parasite control (without any treatment), and pentamidine. ***P < 0.001 in comparison to parasite control. (b). Growth Reversibility Analysis. L. donovani promastigotes (2 × 106 ml−1) were incubated with ALE, ASE, pentamidine and DMSO (solvent control, 0.5%) and growth reversal after drug treatment was analyzed as described in Methods. ***P < 0.001 with respect to parasite control.Back to article page