Figure 5From: Apoptosis mediated leishmanicidal activity of Azadirachta indica bioactive fractions is accompanied by Th1 immunostimulatory potential and therapeutic cure in vivo Impact of ALE and ASE on cellular events leading to induction of apoptosis. (a). Effect of A. indica fractions on Ψm in L. donovani promastigotes. Untreated and treated parasites (2 × 106 ml−1, 500 μg ml−1) were incubated for 72 h and stained with JC-1 and acquired in a flow cytometer. Red and green fluorescence positive cells were separately gated and their ratio was represented as relative Ψm values. ***P < 0.001, **P < 0.01 in comparison to parasite control. (b). ROS generation in L. donovani promastigotes. Leishmania parasites (2 × 106 ml−1) were grown in the presence or absence of bioactive fractions and pentamidine (500 μg ml−1) for 72 h and stained with H2DCFDA. Data was analyzed in the form of histograms where a shift in MFI in P2 gated region was recorded.Back to article page