Table 1 Experimental design for in vivo studies

Group 1 (Normal)

Normal control/ Naïve mice (Administered PBS only)

Group 2 (INF)

Infected control (BALB/c mice infected with L. donovani promastigotes and administered PBS)

Group 3 (VC)

Vehicle control (0.5% DMSO in PBS, the highest concentration used to dissolve the fractions/compounds)

Group 4 (AmB)

Amphotericin B administered intravenously (i.v.) at (5 mg/kg bw)

Group 5 (ALE100)

ALE administered intraperitoneally (i.p.) at 100 mg/kg bw

Group 6 (ALE 200)

ALE administered i.p. at 200 mg/kg bw

Group 7 (ASE100)

ASE administered i.p. at 100 mg/kg bw

Group 8 (ASE200)

ASE administered i.p. at 200 mg/kg bw