Cell migration by wound-healing assay. Confluent cell cultures were wounded post-treatment and migration distances were measured at 8 hours. The experiment was carried out in canine endothelial (A) and smooth muscle cells (B) untreated or treated with 1 μg/ml of DiES + 10 μg/ml of PLG, 1 μg/ml of DiES, 10 μg/ml of PLG, or with 1 μg/ml of DiES + 10 μg/ml of PLG + 50 mM of the εACA. The results were expressed as percentage of the migration ability of the negative control cells (100%). Each point is the mean ± SD from three independent experiments. The asterisk (*) designates significant (p < 0.05) differences between DiES + PLG treatment and control groups.