Effect of DiES on the expression of tPA (A), uPA (B), annexin A2 (C) and PAI-1 (D) in canine vascular endothelial (EC) and smooth muscle cells (SMC). Protein extracts from lysed DiES untreated or treated confluent cell cultures were analyzed by Western blot for tPA, uPA, annexin A2 and PAI-1. α-tubulin served as a protein control. Results were expressed as the mean ± SD of at least 3 independent experiments. The asterisk (*) designates significant (p < 0.05) differences from control cells. (Gray bars) Non-treated control cells. (Orange bars) Stimulated endothelial or smooth muscle cells with 1 μg/ml of DiES.