Skip to main content

Table 1 Primers used for qRT-PCR analysis of miRNA expression

From: Altered levels of circulating miRNAs are associated Schistosoma japonicum infection in mice

MicroRNAs

Primer sequences 5'-3'

Annealing temperature (°C)

Reverse transcription

  

let-7b-3p

GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGGGAAG

 

miR-1194

GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAGGATC

 

miR-134-5p

GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCCCCTC

 

miR-1981-3p

GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGTCAAA

 

miR-210-5p

GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCAGTGT

 

miR-92a-2-5p

GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGTAATG

 

miR-542-3p

GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTTTCGT

 

miR-706

GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTTTTTT

 

Quantitative RT-PCR

  

let-7b-3p

F: ATCGTACGTGGGCTATACAAC

61.5

miR-1194

F: ATCGTACGTGGGGAATGAGTA

57

miR-134-5p

F: ATCGTACGTGGGTGTGACTGG

61.5

miR-1981-3p

F: ATCGTACGTGGGCATCTAACC

58

miR-210-5p

F: ATCGTACGTGGGAGCCACTGC

61.5

miR-92a-2-5p

F: ATCGTACGTGGGAGGTGGGGA

62

miR-542-3p

F:ATCGTACGTGGGTGTGACAGA

57.5

miR-706

F:ATCGTACGTGGGAGAGAAACC

57.5

Common reverse primer

R: GCAGGGTCCGAGGTATTC

 

GAPDH

F: CATGGCCTTCCGTGTTCCTA

62

GAPDH

R: CCTGCTTCACCACCTTCTTGAT

 
  1. F: forward, R: reverse.