From: Altered levels of circulating miRNAs are associated Schistosoma japonicum infection in mice
MicroRNAs | Primer sequences 5'-3' | Annealing temperature (°C) |
---|---|---|
Reverse transcription | Â | Â |
let-7b-3p | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGGGAAG | Â |
miR-1194 | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAGGATC | Â |
miR-134-5p | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCCCCTC | Â |
miR-1981-3p | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGTCAAA | Â |
miR-210-5p | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCAGTGT | Â |
miR-92a-2-5p | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGTAATG | Â |
miR-542-3p | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTTTCGT | Â |
miR-706 | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTTTTTT | Â |
Quantitative RT-PCR | Â | Â |
let-7b-3p | F: ATCGTACGTGGGCTATACAAC | 61.5 |
miR-1194 | F: ATCGTACGTGGGGAATGAGTA | 57 |
miR-134-5p | F: ATCGTACGTGGGTGTGACTGG | 61.5 |
miR-1981-3p | F: ATCGTACGTGGGCATCTAACC | 58 |
miR-210-5p | F: ATCGTACGTGGGAGCCACTGC | 61.5 |
miR-92a-2-5p | F: ATCGTACGTGGGAGGTGGGGA | 62 |
miR-542-3p | F:ATCGTACGTGGGTGTGACAGA | 57.5 |
miR-706 | F:ATCGTACGTGGGAGAGAAACC | 57.5 |
Common reverse primer | R: GCAGGGTCCGAGGTATTC | Â |
GAPDH | F: CATGGCCTTCCGTGTTCCTA | 62 |
GAPDH | R: CCTGCTTCACCACCTTCTTGAT | Â |