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Figure 4 | Parasites & Vectors

Figure 4

From: Inflammatory responses and intestinal injury development during acute Trypanosoma cruzi infection are associated with the parasite load

Figure 4

Immunohistochemical analysis of T. cruzi in the intestinal tissues of different groups. Amastigote nest positivity was evaluated according to the presence of intestinal tissue nests in 10 animals per group (low, medium, and high doses). Immunohistochemistry was performed with antibodies against T. cruzi, and the subsequent immune complexes were visualized with the substrate diaminobenzidine. Images were obtained using a 20× objective lens. Antigenic staining of amastigote nests was observed in the intestinal tissues, as indicated by arrows (A). A comparison of nest positivity according to group (in percentages) at 7 and 14 days after infection (B). The groups were compared on the different days and between the different days. The letters a, b and c indicate statistically significant differences between them (p < 0.05). Chi-squared test was used here.

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