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Figure 5 | Parasites & Vectors

Figure 5

From: Inflammatory responses and intestinal injury development during acute Trypanosoma cruzi infection are associated with the parasite load

Figure 5

Determination of cytokine concentrations in the intestinal tissues. The TNF-α (A), IFN-γ (B), IL-2 (C), IL-6 (D), IL-17 (E), and IL-10 (F) concentrations in intestinal tissue slices were determined on days 0 (without infection), 7, and 14 after T. cruzi infection in mice that received low, medium, and high parasite doses. The samples were obtained by homogenizing tissues in a protease inhibitor buffer, followed by centrifugation at 300 × g for 15 min. A cytokine bead array was used for cytokine detection and quantification, and the samples were analyzed by flow cytometry with the CellQuest software as described in the Materials and Methods. The groups were evaluated on separate days. * indicates a significant (p < 0.05) difference between the groups. Parametric tests were used for analysis to time 0 of all cytokines, to time 7 of IL-10, and to time 14 of IL-10, TNF- α, IL-6 and IL-2, and nonparametric tests for other ratings.

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