Table 1 Apparent prevalence and infection intensity with genotypes of Theileria orientalis complex in New Zealand outbreaks
Region | No. of farms | Farm types ( n ) a | Disease groups b | No. of Samples tested | Test-positive by Ikeda TaqMan® qPCR % (proportion) | Test-positive by PCR-HRM % (proportion) | Test-positive by MT PCR % (proportion) | Apparent prevalence of genotype % (proportion) | Mean intensity of infection by genotype (mean no. of DNA copies) | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
ikeda | chitose | buffeli | type 5 | ikeda | chitose | buffeli | type 5 | ||||||||
North Island | |||||||||||||||
Auckland | 13 | Beef (6)/Dairy (4) | NR/ MO | 17 | 94.1 (16/17) | 100 (17/17) | 100 (17/17) | 94.1 (16/17) | 70.6 (12/17) | 100 (17/17) | 29.4 (5/17) | 381,134 | 15,466 | 209,763 | 10,939 |
East Cape | 8 | Beef (7)/Dairy (1) | NR/MO | 18 | 44.4 (8/18) | 100 (18/18) | 100 (18/18) | 44.4 (8/18) | 77.8 (14/18) | 83.3 (15/18) | 22.2 (4/18) | 64,727 | 27,164 | 68,263 | 9 |
Northland | 14 | Beef (5)/Dairy (9) | NR/MO/ANT | 32 | 100 (32/32) | 100 (32/32) | 100 (32/32) | 100 (32/32) | 87.5 (28/32) | 100 (32/32) | 53.1 (17/32) | 237,979 | 129,868 | 159,724 | 15,823 |
Waikato | 62 | Beef (5)/Dairy (56) | NR/MO/ANT/SO | 87 | 90.8 (79/87) | 98.9 (86/87) | 98.9 (86/87) | 90.7 (78/86) | 31.4 (27/86) | 100 (86/86) | 3.5 (3/86) | 420,479 | 57,012 | 186,854 | 859 |
Total | 97 | Beef (23)/Dairy (70) | 154 | 87.7 (135/154) | 99.4 (153/154) | 99.4 (153/154) | 87.6 (134/153) | 52.9 (81/153) | 98.0 (150/153) | 19.0 (29/153) | 349,271 | 70,684 | 170,786 | 11,252 | |
South Island | |||||||||||||||
Southland | 9 | Beef (9) | NR | 88 | 0% (0/88) | 2.3% (2/88) | 2.3% (2/88) | 0 | 100 (2/2) | 100 (2/2) | 100 (2/2) | 0 | 6,916 | 7,694 | 22 |