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Table 1 Apparent prevalence and infection intensity with genotypes of Theileria orientalis complex in New Zealand outbreaks

From: Comparison of the performance of three PCR assays for the detection and differentiation of Theileria orientalis genotypes

Region No. of farms Farm types ( n ) a Disease groups b No. of Samples tested Test-positive by Ikeda TaqMan® qPCR % (proportion) Test-positive by PCR-HRM % (proportion) Test-positive by MT PCR % (proportion) Apparent prevalence of genotype % (proportion) Mean intensity of infection by genotype (mean no. of DNA copies)
ikeda chitose buffeli type 5 ikeda chitose buffeli type 5
North Island                
Auckland 13 Beef (6)/Dairy (4) NR/ MO 17 94.1 (16/17) 100 (17/17) 100 (17/17) 94.1 (16/17) 70.6 (12/17) 100 (17/17) 29.4 (5/17) 381,134 15,466 209,763 10,939
East Cape 8 Beef (7)/Dairy (1) NR/MO 18 44.4 (8/18) 100 (18/18) 100 (18/18) 44.4 (8/18) 77.8 (14/18) 83.3 (15/18) 22.2 (4/18) 64,727 27,164 68,263 9
Northland 14 Beef (5)/Dairy (9) NR/MO/ANT 32 100 (32/32) 100 (32/32) 100 (32/32) 100 (32/32) 87.5 (28/32) 100 (32/32) 53.1 (17/32) 237,979 129,868 159,724 15,823
Waikato 62 Beef (5)/Dairy (56) NR/MO/ANT/SO 87 90.8 (79/87) 98.9 (86/87) 98.9 (86/87) 90.7 (78/86) 31.4 (27/86) 100 (86/86) 3.5 (3/86) 420,479 57,012 186,854 859
Total 97 Beef (23)/Dairy (70)   154 87.7 (135/154) 99.4 (153/154) 99.4 (153/154) 87.6 (134/153) 52.9 (81/153) 98.0 (150/153) 19.0 (29/153) 349,271 70,684 170,786 11,252
South Island                
Southland 9 Beef (9) NR 88 0% (0/88) 2.3% (2/88) 2.3% (2/88) 0 100 (2/2) 100 (2/2) 100 (2/2) 0 6,916 7,694 22
  1. a n denotes the number of farms sampled. For some farms the farm type (dairy or beef) information were not collected.
  2. bDisease groups were categorized according to clinical signs and haematological parameters. ‘NR’, ‘ANT’, ‘MO’ and ‘SO’ denote no disease reported, anaemia negative Theileria (i.e., anaemia not observed but Theileria piroplasms were observed on blood smears), moderate outbreak (i.e., more than one animal with anaemia) and severe outbreak (i.e., with multiple deaths), respectively.