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Table 5 Diagnostic test performance of MT PCR and Ikeda TaqMan® qPCR

From: Comparison of the performance of three PCR assays for the detection and differentiation of Theileria orientalis genotypes

Outcome Median (95% PI) a
MT PCR  
Diagnostic sensitivity (%) 98.0 (94.6, 99.6)
Diagnostic specificity (%) 98.9 (96.4, 99.8)
Ikeda TaqMan® qPCR  
Diagnostic sensitivity (%) 97.1 (94.2, 98.8)
Diagnostic specificity (%) 97.4 (95.1, 98.8)
Estimated true prevalence
Group 1 (%) 88.1 (82.5, 93.1)
Group 2 (%) 0 (0, 0.12)
Agreement between tests  
PABAK in group 1 94.0 (89.0, 97.2)
PABAK in group 2 94.1 (88.0, 97.7)
Conditional dependence between tests b
ρ+ 0.371 (0.004, 0.764)
ρ- 0.226 (-0.006, 0.674)
Model fit  
pD 7.6
DIC 28.4
  1. The diagnostic sensitivity of the MT PCR was defined based on a cut-off of ≥ 1 DNA copies.
  2. aPI, probability interval.
  3. b ρ+ is the conditional correlation between MT and Ikeda TaqMan® qPCRs for infected animals, and ρ- is the conditional correlation for uninfected animals.
  4. PABAK = prevalence-adjusted bias-adjusted Cohen’s kappa (ĸ) statistic.
  5. pD = effective dimension (model complexity).
  6. DIC = Deviance information criterion, a generalisation of Akaike’s Information Criterion (AIC).