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Figure 2 | Parasites & Vectors

Figure 2

From: Transmembrane protein 63A is a partner protein of Haemonchus contortus galectin in the regulation of goat peripheral blood mononuclear cells

Figure 2

TMEM63A localized to the cell surface in PBMCs. Goat PBMC were first fixed and then permeabilized with detergent prior to IF analysis. Then, cells were incubated with rat anti-TMEM63A-NO IgG (TMEM63A) or negative rat IgG (Control). DIO (green), DAPI (blue) and Cy3-conjugated secondary antibodies (red) were used for triple staining. (A and D): cell membrane (green) and nuclei (blue) staining of cells; (B and E): staining of target proteins (red); (C and F): a merged image of the three colors. TMEM63A only localized to the cell membranes. Scale bars represent 5000 nm.

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