Figure 6

Knockdown of the TMEM63A gene and rHco-gal-m-treatment affected the PBMC nitrite oxide production. PBMC can be share as blank group (group 1), ns siRNA group (group 2), ns siRNA/g group (group 3), 63A siRNA group (group 4) and 63A siRNA/g group (group 5), which were incubated with equal volume reduced serum medium (group 1), ns siRNA (group 2 and 3) and TMEM63A-siRNA-1 (group 4 and 5) for 60 h. RHco-gal-m in all RNA interference groups (group 3 and 5) was added 12 h before the end of RNA interference period. The nitrite concentration in the PBMCs was measured by using the Griessassay and used as an indicator of nitric oxide production by the PBMCs. Results presented here were collected from one independent experiment and were representative of three independent experiments. Data were represented as mean ± SD, n = 4; an asterisk and a capped line designates two groups differ significantly (p < 0.001).