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Table 1 Specifications of the PCR assays used in the study

From: Epidemiology of Trypanosoma evansi and Trypanosoma vivax in domestic animals from selected districts of Tigray and Afar regions, Northern Ethiopia

Taxon Target gene Primers Primer sequences Amplicon length Reference
Vertebrates Cytochrome b L14841 5′-CCATCCAACATCTCAGCATGATGAAA-3′ 400 bp Adapted from [73]
H15149 5′-GCCCCTCAGAATGATATTTGTCCTCA-3′
T. evansi Type A VSG RoTat 1.2 RoTat1.2-F 5′-GCGGGGTGTTTAAAGCAATA-3′ 205 bp Adapted from [37]
RoTat1.2-R 5′-ATTAGTGCTGCGTGTGTTCG-3′
T. evansi Type B minicircle EVAB-1 5′-ACAGTCCGAGAGATAGAG-3′ 436 bp Adapted from [34]
EVAB-2 5′-CTGTACTCTACATCTACCTC-3′
T. vivax Proline racemase TvPRAC-F 5′ CGCAAGTGGACCGTTCGCCT- 3′ 239 bp Adapted from [56]
TvPRAC-R 5′ ACGCGGGGCGAACAGAAGTG-3′
Diverse Trypanosoma species ITS-1 ITS-1 F 5′-TGTAGGTGAACCTGCAGCTGGATC-3′ T. vivax 150 bp, T. theileri 350 bp, Trypanozoon 450 bp, T. congolense 650 bp [44]
ITS-1 R 5′-CCAAGTCATCCATCGCGACACGTT- 3′
  1. bp: base pairs.