Table 1 Specifications of the PCR assays used in the study

Vertebrates

Cytochrome b

L14841

5′-CCATCCAACATCTCAGCATGATGAAA-3′

400 bp

Adapted from [73]

H15149

5′-GCCCCTCAGAATGATATTTGTCCTCA-3′

T. evansi Type A

VSG RoTat 1.2

RoTat1.2-F

5′-GCGGGGTGTTTAAAGCAATA-3′

205 bp

Adapted from [37]

RoTat1.2-R

5′-ATTAGTGCTGCGTGTGTTCG-3′

T. evansi Type B

minicircle

EVAB-1

5′-ACAGTCCGAGAGATAGAG-3′

436 bp

Adapted from [34]

EVAB-2

5′-CTGTACTCTACATCTACCTC-3′

T. vivax

Proline racemase

TvPRAC-F

5′ CGCAAGTGGACCGTTCGCCT- 3′

239 bp

Adapted from [56]

TvPRAC-R

5′ ACGCGGGGCGAACAGAAGTG-3′

Diverse Trypanosoma species

ITS-1

ITS-1 F

5′-TGTAGGTGAACCTGCAGCTGGATC-3′

T. vivax 150 bp, T. theileri 350 bp, Trypanozoon 450 bp, T. congolense 650 bp

[44]

ITS-1 R

5′-CCAAGTCATCCATCGCGACACGTT- 3′