Epidemiology of Trypanosoma evansi and Trypanosoma vivax in domestic animals from selected districts of Tigray and Afar regions, Northern Ethiopia

Table 1 Specifications of the PCR assays used in the study

Taxon	Target gene	Primers	Primer sequences	Amplicon length	Reference
Vertebrates	Cytochrome b	L14841	5′-CCATCCAACATCTCAGCATGATGAAA-3′	400 bp	Adapted from [73]
Vertebrates	Cytochrome b	H15149	5′-GCCCCTCAGAATGATATTTGTCCTCA-3′	400 bp	Adapted from [73]
T. evansi Type A	VSG RoTat 1.2	RoTat1.2-F	5′-GCGGGGTGTTTAAAGCAATA-3′	205 bp	Adapted from [37]
T. evansi Type A	VSG RoTat 1.2	RoTat1.2-R	5′-ATTAGTGCTGCGTGTGTTCG-3′	205 bp	Adapted from [37]
T. evansi Type B	minicircle	EVAB-1	5′-ACAGTCCGAGAGATAGAG-3′	436 bp	Adapted from [34]
T. evansi Type B	minicircle	EVAB-2	5′-CTGTACTCTACATCTACCTC-3′	436 bp	Adapted from [34]
T. vivax	Proline racemase	TvPRAC-F	5′ CGCAAGTGGACCGTTCGCCT- 3′	239 bp	Adapted from [56]
T. vivax	Proline racemase	TvPRAC-R	5′ ACGCGGGGCGAACAGAAGTG-3′	239 bp	Adapted from [56]
Diverse Trypanosoma species	ITS-1	ITS-1 F	5′-TGTAGGTGAACCTGCAGCTGGATC-3′	T. vivax 150 bp, T. theileri 350 bp, Trypanozoon 450 bp, T. congolense 650 bp	[44]
Diverse Trypanosoma species	ITS-1	ITS-1 R	5′-CCAAGTCATCCATCGCGACACGTT- 3′		[44]

ISSN: 1756-3305