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Figure 1 | Parasites & Vectors

Figure 1

From: Entamoeba histolytica: protein arginine transferase 1a methylates arginine residues and potentially modify the H4 histone

Figure 1

Identification of an epigenetic mark equivalent to H4R3me2 in E. histolytica . (A) Alignment of the H4 N-termini of Homo sapiens (Hs) and E. histolytica (Eh). (*) Identical residues. (.) Similar residues. Rectangles indicate arginine and lysine residues susceptible to methylation. (B) Western blot using α-H4R3me2 commercial antibody. (N) Nuclear basic proteins and (C) cytoplasmic extracts derived from E. histolytica trophozoites and HeLa human cells. As control for cellular fractions, membranes were incubated with α-EhCPADH, antibody against a cytoplasmic protein of E. histolytica and with α-HsH4, antibody against unmethylated human H4, used as a nuclear marker.

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