Figure 7

Detection of the recombinant GST/EhPRMT1a by α-PRMT1 and oligomerization of EhPRMT1a. (A-C) Purified GST and GST/EhPRMT1a recombinant proteins were separated in 12% SDS-PAGE and transferred to nitrocellulose filters. (A) SDS-PAGE stained with Coomassie blue. (B, C) Western blot assays using (B) α-GST or (C) α-HsPRMT1 antibodies. (D) Recombinant EhPRMT1a without the GST tag was incubated in the absence (−) or in the presence (+) of 0.2% glutaraldehyde for 20 min at 25°C prior to SDS-PAGE and silver staining. Bands corresponding to monomers, dimers and tetramers are indicated.