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Table 1 Primers used to amplify the Ehprmt genes

From: Entamoeba histolytica: protein arginine transferase 1a methylates arginine residues and potentially modify the H4 histone

Gene

Primer sequences

EHI_105780 ( Ehprmt1a )*

S: 5′-cccccggatccatgtcaatggaacaactc-3′

AS: 5′-cccccctcgagttaatttaaatgatattctattttgt-3′

EHI_152460 ( Ehprmt1b )

S: 5′-atggaagaatgcaaaccaac-3′

AS: 5′-tcaattcatatgataaacttggtcat 3′

EHI_202470 ( Ehprmt1c )

S: 5′-atggaatcaaaacaatttg-3′

AS: 5′-ttattgcatttgatatttcatt-3′

EHI_159780 ( Ehprmt-a )

S: 5′-atgaaagaaatgaagagaagttt-3′

AS: 5′-ttaaaattcatattgatattttcctg-3′

  1. S: sense primer. AS: anti-sense primer.
  2. *Sense and anti-sense primers of Ehprmt1a contains at their 5′-end the recognition sites for BamH1 and Xho1 (italic), respectively.
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Parasites & Vectors

ISSN: 1756-3305

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