Figure 2

Alcian blue and periodic acid Schiff (PAS) stained histological sections through the skeletal muscle of Perca fluviatilis L. (a) Larval specimens of Eustrongylides sp. (arrow heads) within the muscle of P. fluviatilis where the nematode has replaced large portions of the skeletal muscle resulting in compression (arrow); scale bar = 200 μm. (b) PAS-positive macrophage aggregates (arrows) and multifocal fibro-epithelioid granulomata (arrow heads) are evident within the thickness of the capsule enclosing the Eustrongylides sp. larva. Note the PAS-positive necrotic material (asterisk); scale bar = 100 μm. (c) Histological section through nematode-infected muscle that has been stained with a PCNA-antibody. Positive fibroblasts (arrows) are scattered among the collagen fibres. Part of the nematode (asterisk) can be seen compressing the muscle tissues; scale bar = 20 μm. (d) PCNA-positive satellite cells (arrows) in the outer part of the myotubes (asterisk) can be seen; scale bar = 10 μm.