Characterization of anti-glycan natures on monoclonal antibodies. A. The bindings of monoclonal antibodies with SjEA were measured by ELISA and presented as mean ± SEM of the OD values from triplicate wells at different dilutions. B. Comparison of bindings of monoclonal IgGs with SjEA versus with pSjEA by ELISA. t test was performed on mean values obtained from triplicate wells from single experiment with *** as p < 0.001. C. Inhibitory effects on bindings of monoclonal antibodies by egg antigens devoid of proteins. Similar methods were applied as in Figure 1C and D. Data shown were mean ± SEM values from triplicate wells. D. Mock- treated (lane 1) or PNGase F-treated egg antigens (lane 2) were detected by monoclonal antibodies with western blot analysis. HRP-ConA was used to test the effect of PNGase F digestion on SjEA and anti-GST was used as loading control. Shown was a representative of at least three separate experiments.