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Table 1 Primers used to detect Babesia and other tick-borne pathogens by PCR

From: Babesia spp. and other pathogens in ticks recovered from domestic dogs in Denmark

Pathogen PCR principle Gene target Primer name Primer sequence Reference
Babesia spp. PCR and sequencing 18S rRNA gene BJ1 5′-GTCTTGTAATTGGAATGATGG-3′ [21]
BN2 5′-TAGTTTATGGTTAGGACTACG-3′
Bartonella henselae   Riboflavin synthase gene BartF 5′-ACGGATATCGGTTGTGTTGAGGA-3′ Present study (modified from [22])
PBH-R2 5′-AGGTATAAAACGCTTTGGTACTTGTAGG-3′
Bartonella quintana   Riboflavin synthase gene BartF 5′-ACGGATATCGGTTGTGTTGAGGA-3′ Present study
PBQR 5′-TTACAATAAAGGGCGTGATGAATTTTGTT-3′
Borrelia burgdorferi Nested PCR Flagellin gene Outer1 5′-AATGAATTGGCAGTTCAATC-3′ [23,24]
Outer2 5′-GCATTTTCWATTTTAGCAAGTGATG-3′
Inner1 5′-ACATATTCAGATGCAGACAGAGGTTCTA-3′
Inner2 5′-GAAGGTGCTGTAGCAGGTGCTGGCTGT-3′
Francisella tularensis   peptidyl-prolyl trans isomerase and RNA helicase genes Ft-M19 5′-CCAGTACAAACTCAATTTGGTTATCATC-3′ [25]
Ft-M19R 5′-TAGTTTCAGAATTCATTTTTGTCCGTAA-3′
Rickettsia spp.   16S rRNA gene 16SF2 5′-ACGCTATCGGTATGCTTAACACAT-3′ Present study
16SR2 5′-CAACTTACTAAACCGCCTACGCACT-3′
Candidatus Neoehrlichia mikurensis   16S rRNA gene NEO-M140F 5′-ATGGAATAGCTGTTAGAAATGAC-3′ Present study
NEO-630R 5′-CTATCCTCTCTCGATCTCTAGTTT-3′