Table 1 Primers of ovine beta-actin and five immune-relevant genes selected for quantitative RT-PCR validation
Acronym | Gene name | Primer sequence (5′ – 3′) | GenBank number | Product size (bp) |
|---|---|---|---|---|
Beta-actin | Beta-actin | GGCAGGTCATCACCATCGGCAAT GCGTAGAGGTCTTTGCGGATGT | U39357.1 | 151 |
IL-8 | Interleukin 8 | AAAAGTGGGTGCAGAAGG CTCAAGGCACTGAAGTAGAT | NM_001009401.1 | 163 |
FCER1G | Fc fragment of IgE, high affinity I, receptor for; gamma polypeptide | GACCCAGGAGACTTATGAGACC GCGTATGTGATGCCAACC | NM_174537.2 | 123 |
IGLL1 | immunoglobulin lambda-like polypeptide 1 | CTCCAAACAGAGCAACAGC TGAGGGCTTCACTGTCTTC | NM_001083800.1 | 136 |
CXCL2 | chemokine (C-X-C motif) ligand 2 | AGGACTTGATGTGCTGGACT CAGGACTGGGTTATGTTTG | NM_174299.2 | 139 |
OVAR | MHC class I antigen | TGTTGCGGAGGCAGAAAGG ATGTGCCTTTGGAGGGTCTGC | NM_001130934.1 | 112 |