Figure 4

Effect on cyclic AMP levels. A whole body homogenate from third instar Aedes aegypti larvae was assayed for adenylate cyclase activity, as described in ‘Materials and Methods’ section, in the presence of 100 μM of four flavonoids, four unsaturated fatty acids, and three saturated fatty acids. The effects of the test compounds on cAMP levels of the homogenate were compared with those induced by octopamine (100 μM) alone. Data were expressed as nmol/4.22 μg protein. Each bar represents the mean ± standard error of duplicate samples of three independent experiments. (^* p = 0.05, according to Bonferroni multiple-comparison method).