Wolbachia density in salivary gland, midgut and body of wMel.F mosquitoes. Significantly more Wolbachia was found in the remainder of the mosquito body compared to the salivary glands and midguts (P < 0.0001). Salivary glands had significantly more Wolbachia than the midguts (P = 0.0009). Y-axis shows ratio of wsp/Rps17. Letters denote distinct statistical groups and error bars are standard error of the mean of 16–17 biological replicates.