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Figure 2 | Parasites & Vectors

Figure 2

From: Effect of repeat human blood feeding on Wolbachia density and dengue virus infection in Aedes aegypti

Figure 2

DENV-2 RNA copies in salivary glands of wMel.F and Wildtype mosquitoes. In replicate A, wMel.F mosquitoes fed twice on human bloodmeal (Fed 2x) prior to being infected had complete viral blocking 10–11 dpi. This was significantly lower (P = 0.02) than the wMel.F mosquitoes which were not fed on human bloodmeal (Unfed) prior to being infected. The Wildtype mosquitoes which had two repeat human bloodmeals (Fed 2x) had significantly lower copies of DENV-2 RNA compared to those which did not have human bloodmeal (Unfed) (P = 0.005) and those which had only one human bloodmeal (Fed 1x) (P = 0.005). In replicate B, there was complete DENV-2 blocking in the wMel.F salivary glands in all three treatment groups and in the Wildtype mosquitoes, the effect of repeat blood feeding was not significant. Comparisons were made within mosquito lines and across treatment groups. Letters represent distinct statistical groups. Bars denote medians and each point represents individual salivary gland.

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