Wolbachia density in wMel.F mosquito midguts. In replicate A, midgut Wolbachia density in wMel.F mosquitoes decreased in mosquitoes fed twice on human bloodmeal (Fed 2x) before being challenged with DENV-2 compared to those which were not blood fed (Unfed) (P = 0.002) and those fed only once on human blood (Fed 1x) (P = 0.03). However in replicate B, there was no significant change in Wolbachia density across treatment groups. Y-axis shows ratio of wsp/Rps17. Letters represent distinct statistical groups. Error bars are standard error of the mean of 14-21individual midguts.