Figure 5From: Effect of repeat human blood feeding on Wolbachia density and dengue virus infection in Aedes aegypti Wolbachia density in wMel.F mosquito midguts. In replicate A, midgut Wolbachia density in wMel.F mosquitoes decreased in mosquitoes fed twice on human bloodmeal (Fed 2x) before being challenged with DENV-2 compared to those which were not blood fed (Unfed) (P = 0.002) and those fed only once on human blood (Fed 1x) (P = 0.03). However in replicate B, there was no significant change in Wolbachia density across treatment groups. Y-axis shows ratio of wsp/Rps17. Letters represent distinct statistical groups. Error bars are standard error of the mean of 14-21individual midguts.Back to article page