DENV-2 RNA copies in the body of wMel.F and Wildtype mosquitoes. In replicate A, wMel.F mosquitoes that fed twice on human bloodmeal (Fed 2x) prior to being infected had complete viral blocking in the remainder of the mosquito body 10–11 dpi. This was significantly lower (P = 0.02) than the wMel.F mosquitoes which were not fed human bloodmeal (Unfed) prior to being infected. Repeat blood feeding did not have a significant effect on DENV-2 RNA copies in the remains of the Wildtype mosquito body. In replicate B, there was no significant change in DENV-2 RNA copies between treatment groups in both wMel.F and Wildtype mosquitoes. Letters represent distinct statistical groups. Bars denote medians and each point represents an individual carcass/remainder of the mosquito body.