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Table 2 Gene expression stability rankings for the different physiological conditions studied in female antennae

From: Evaluation of reference genes for insect olfaction studies

Ranking position Effect of nutrition in larvae Effect of nutrition in adults Imaginal moult effect Adult maturation effect
geNorm NormFinder NormFinder w geNorm NormFinder NormFinder w geNorm NormFinder NormFinder w geNorm NormFinder NormFinder w
1 Tub/GAPDH Tub Tub G6PDH/GAPDH Tub Tub G6PDH/SDH SDH SDH Sp/GAPDH Tub Tub
(0.215) (0.079) (0.083) (0.327) (0.186) (0.169) (0.387) (0.193) (0202) (0.345) (0.271) (0.268)
2 - SDH G6PDH - G6PDH SDH - GAPDH GAPDH - G6PDH G6PDH
(0.155) (0.125)   (0.256) (0.232)   (0.217) (0.208)   (0.284) (0.326)
3 GST GST GST Tub SDH G6PDH GAPDH G6PDH Act G6PDH SDH SDH
(0.251) (0.171) (0.133) (0.352) (0.269) (0.253) (0.476) (0.431) (0.323) (0.393) (0.3) (0.326)
4 G6PDH G6PDH SDH eIF-1a GAPDH GAPDH Act Act G6PDH Tub Sp GST
(0.272) (0.2) (0.158) (0.388) (0.331) (0.261) (0.543) (0.492) (0.478) (0.42) (0.515) (0.403)
5 SDH GAPDH GAPDH SDH eIF-1a eIF-1a Sp Sp Sp SDH GST Sp
(0.301) (0.268) (0.18) (0.407) (0.35) (0.267) (0.578) (0.607) (0.570) (0.515) (0.533) (0.51)
6 eIF-1a eIF-1a Act Sp Sp Sp GST Tub Tub GST GAPDH GAPDH
(0.348) (0.494) (0.306) (0.444) (0.474) (0.288) (0.629) (0.704) (0.692) (0.56) (0.568) (0.548)
7 Act Act eIF-1a GST GST GST Tub GST GST eIF-1a eIF-1a eIF-1a
(0.406) (0.502) (0.333) (0.485) (0.508) (0.305) (0.708) (0.823) (0.811) (0.618) (0.589) (0.559)
8 Sp Sp Sp Act Act Act eIF-1a eIF-1a eIF-1a Act Act Act
(0.523) (0.888) (0.395) (0.529) (0.575) (0.345) (0.799) (0.997) (0.955) (0.736) (1.052) (0.902)
    Best combination    Best combination    Best combination    Best combination
GP6PDH–GST    G6PDH–eIF-1a    GAPDH–SDH    G6PDH–SDH
(0.068)    (0.101)    (0.077)    (0.079)
  1. geNorm and Normfinder rankings were built using M-value and stability value, respectively. The Normfinder ranking did not take experimental groups into account, while the Normfinderw one considered them for the analysis of expression stability.