Evaluation of reference genes for insect olfaction studies

Parasites & Vectors

Table 3 Gene expression stability rankings for the different physiological conditions studied in male antennae

Ranking position	Effect of nutrition in larvae			Effect of nutrition in adults			Imaginal moult effect			Adult maturation effect
Ranking position	geNorm	NormFinder	NormFinder ^w	geNorm	NormFinder	NormFinder ^w	geNorm	NormFinder	NormFinder ^w	geNorm	NormFinder	NormFinder ^w
1	G6PDH/GAPDH	SDH	G6PDH	SDH/Tub	SDH	SDH	SDH/GAPDH	Act	SDH	GST/G6PDH	G6PDH	Sp
1	(0.14)	(0.051)	(0.141)	(0.306)	(0.259)	(0.155)	(0.332)	(0.423)	(0.283)	(0.378)	(0.421)	(0.296)
2		G6PDH	SDH		GAPDH	Act	-	GAPDH	GAPDH	-	Sp	GST
2		(0.128)	(0.164)	-	(0.265)	(0.195)		(0.424)	(0.293)		(0.447)	(0.334)
3	Tub	GAPDH	GAPDH	GAPDH	Tub	G6PDH	Act	G6PDH	Sp	eIF-1a	Tub	G6PDH
3	(0.215)	(0.162)	(0.18)	(0.361)	(0.298)	(0.212)	(0.419)	(0.426)	(0.313)	(0.494)	(0.452)	(0.391)
4	SDH	Tub	Tub	Act	Act	Tub	G6PHD	SDH	Act	Sp	eIF-1a	SDH
4	(0.227)	(0.195)	(0.224)	(0.39)	(0.331)	(0.214)	(0.536)	(0.432)	(0.410)	(0.564)	(0.454)	(0.399)
5	GST	GST	GST	G6PDH	G6PDH	GAPDH	GST	Sp	G6PDH	Tub	GST	Tub
5	(0.319)	(0.35)	(0.344)	(0.423)	(0.375)	(0.244)	(0.578)	(0.492)	(0.426)	(0.599)	(0.511)	(0.411)
6	Act	Act	Sp	GST	GST	GST	Sp	Tub	Tub	SDH	SDH	eIF-1a
6	(0.399)	(0.628)	(0.499)	(0.484)	(0.504)	(0.246)	(0.615)	(0.544)	(0.528)	(0.659)	(0.527)	(0.429)
7	eIF-1a	eIF-1a	Act	eIF-1a	eIF-1a	eIF-1a	Tub	GST	GST	Act	Act	GAPDH
7	(0.486)	(0.697)	(0.577)	(0.52)	(0.519)	(0.325)	(0.665)	(0.631)	(0.617)	(0.687)	(0.634)	(0.543)
8	Sp	Sp	eIF-1a	Sp	Sp	Sp	eIF-1a	eIF-1a	eIF-1a	GAPDH	GAPDH	Act
8	(0.578)	(0.8)	(0.636)	(0.598)	(0.779)	(0.35)	(0.726)	(0.806)	(0.767)	(0.715)	(0.64)	(0.571)
			Best combination			Best combination			Best combination			Best combination
			SDH–GST			Act–SDH			Tub–GST			G6PDH–eIF-1a
			(0.126)			(0.114)			(0.149)			(0.156)

geNorm and Normfinder rankings were built using M-value and stability value, respectively. The Normfinder ranking did not take experimental groups into account, while the Normfinder^w one considered them for the analysis of expression stability.

ISSN: 1756-3305