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Table 3 Gene expression stability rankings for the different physiological conditions studied in male antennae

From: Evaluation of reference genes for insect olfaction studies

Ranking
position
Effect of nutrition in larvae Effect of nutrition in adults Imaginal moult effect Adult maturation effect
geNorm NormFinder NormFinder w geNorm NormFinder NormFinder w geNorm NormFinder NormFinder w geNorm NormFinder NormFinder w
1 G6PDH/GAPDH SDH G6PDH SDH/Tub SDH SDH SDH/GAPDH Act SDH GST/G6PDH G6PDH Sp
(0.14) (0.051) (0.141) (0.306) (0.259) (0.155) (0.332) (0.423) (0.283) (0.378) (0.421) (0.296)
2   G6PDH SDH   GAPDH Act - GAPDH GAPDH - Sp GST
  (0.128) (0.164) - (0.265) (0.195)   (0.424) (0.293)   (0.447) (0.334)
3 Tub GAPDH GAPDH GAPDH Tub G6PDH Act G6PDH Sp eIF-1a Tub G6PDH
(0.215) (0.162) (0.18) (0.361) (0.298) (0.212) (0.419) (0.426) (0.313) (0.494) (0.452) (0.391)
4 SDH Tub Tub Act Act Tub G6PHD SDH Act Sp eIF-1a SDH
(0.227) (0.195) (0.224) (0.39) (0.331) (0.214) (0.536) (0.432) (0.410) (0.564) (0.454) (0.399)
5 GST GST GST G6PDH G6PDH GAPDH GST Sp G6PDH Tub GST Tub
(0.319) (0.35) (0.344) (0.423) (0.375) (0.244) (0.578) (0.492) (0.426) (0.599) (0.511) (0.411)
6 Act Act Sp GST GST GST Sp Tub Tub SDH SDH eIF-1a
(0.399) (0.628) (0.499) (0.484) (0.504) (0.246) (0.615) (0.544) (0.528) (0.659) (0.527) (0.429)
7 eIF-1a eIF-1a Act eIF-1a eIF-1a eIF-1a Tub GST GST Act Act GAPDH
(0.486) (0.697) (0.577) (0.52) (0.519) (0.325) (0.665) (0.631) (0.617) (0.687) (0.634) (0.543)
8 Sp Sp eIF-1a Sp Sp Sp eIF-1a eIF-1a eIF-1a GAPDH GAPDH Act
(0.578) (0.8) (0.636) (0.598) (0.779) (0.35) (0.726) (0.806) (0.767) (0.715) (0.64) (0.571)
    Best combination    Best combination    Best combination    Best combination
SDH–GST    Act–SDH    Tub–GST    G6PDH–eIF-1a
(0.126)    (0.114)    (0.149)    (0.156)
  1. geNorm and Normfinder rankings were built using M-value and stability value, respectively. The Normfinder ranking did not take experimental groups into account, while the Normfinderw one considered them for the analysis of expression stability.