Evaluation of reference genes for insect olfaction studies

Parasites & Vectors

Table 4 Stability values of the female normalization factors

Effect of nutrition in larvae			Imaginal moult effect			Adult maturation effect
geNorm	NormFinder	NormFinder ^w	geNorm	NormFinder	NormFinder ^w	geNorm	NormFinder	NormFinder ^w
Tub-GST-	Tub-GST-	Tub-GST-
G6PDH-GAPDH	G6PDH-GAPDH	G6PDH-GAPDH	SDH-GAPDH	SDH-GAPDH	SDH-GAPDH	G6PDH-Sp-GAPDH	G6PDH-Sp-GAPDH	G6PDH-Sp-GAPDH
(0.103)	(0.047)	(0.065)	(0.241)	(0.193)	(0.076)	(0.257)	(0.35)	(0.366)
Tub-GST-	Tub-GST-	Tub-GST-	G6PDH-SDH	G6PDH-SDH	G6PDH-SDH	G6PDH-Tub-SDH	G6PDH-Tub-SDH	G6PDH-Tub-SDH
G6PDH-SDH	G6PDH-SDH	G6PDH-SDH	(0.191)	(0.217)	(0.251)	(0.303)	(0.116)	(0.038)
(0.103)	(0.0516)	(0.055)
		Best gen			Best gen			Best gen
		Tub-GST-
		G6PDH-SDH			SDH-GAPDH			G6PDH-Tub-SDH
		(0.055)			(0.076)			(0.038)

The Normfinder ranking did not take experimental groups into account, while the Normfinder^w one considered them for the analysis of expression stability. The normalization factor selected is shown in bold for each of the five comparisons.

ISSN: 1756-3305