Small mosquitoes, large implications: crowding and starvation affects gene expression and nutrient accumulation in Aedes aegypti

Table 3 Levels of free amino acids detected in our metabolomic analyses

Compound	SBM v LBM	t-val	q-val	SNBM v LNBM	t-val	q-val
Alanine	144.3	344.5	0.024	0.036	135.1	85.4	0.023	0.036
Aspartic acid	3.9	1.2	0.005	0.036	11.0	0.2	0.001	0.036
Glutamic acid	76.2	69.0	0.613	0.294	22.9	4.4	0.366	0.206
Glycine	252.8	229.2	0.683	0.316	122.2	15.4	0.003	0.036
Histidine	5.0	0.2	0.003	0.036	0.2	0.2	ND	ND
Isoleucine	27.1	115.4	0.006	0.036	20.0	6.6	0.127	0.083
leucine	36.2	79.6	0.101	0.075	24.7	5.7	0.002	0.036
Lysine	22.4	0.2	0.002	0.036	48.5	0.2	0.000	0.036
Methionine	12.5	8.1	0.134	0.083	9.3	0.2	0.012	0.036
Phenylalanine	46.8	42.2	0.813	0.351	31.9	0.2	0.003	0.036
Proline	356.5	962.4	0.174	0.103	33.7	10.2	0.018	0.036
Serine	79.3	196.4	0.133	0.083	62.0	49.7	0.812	0.351
Threonine	64.4	215.9	0.002	0.036	43.6	34.0	0.392	0.211
Tryptophan	38.7	20.6	0.045	0.044	0.2	0.2	ND	ND
Tyrosine	242.6	289.7	0.546	0.272	13.7	54.6	0.001	0.036
Valine	44.8	64.2	0.413	0.214	45.3	16.2	0.021	0.036

Leu, Lys, Phe, Val, Ala, Asp, Glu, Gly and Pro levels were found to be higher in small mosquitoes prior to blood meal, while Tyr was found to be higher in large mosquitoes. Post blood meal Ile, Thr, Trp, and Ala were found to be higher in large mosquitoes while Lys, Asp and His were found to be higher in small mosquitoes.

ISSN: 1756-3305