Figure 1

Oviposition bioassay design. The cups were placed on the dish, which was positioned at the bottom center of the cage. Cups were placed such that each was at an identical distance from the adjacent cup. A: The four oviposition cups contained one of the following media: (i) HCE, (ii) MCE, (iii) cooled boiled water (WAa), or (iv) cooled boiled water (WAb); B: The four oviposition cups contained one of the following media: (i) HCE, (ii) cooled boiled water (WAa), (iii) cooled boiled water (WAb), or (iv) cooled boiled water (WAc); C: The four oviposition cups contained one of the following media: (i) HCEa, (ii) HCEb, (iii) HCEc, and (iv) cooled boiled water (WA).