Figure 3
From: Successful capture of Toxocara canis larva antigens from human serum samples
Detection of L2TES in human sera. 96-well polystyrene plate was coated with PoAb at 5 μg/mL, sera previously treatment with EDTA 100 μL, the MoAb at 10 μg/mL and anti-mouse IgG-HRP diluted 1:8,000.The reaction was revealed with chromogen/substrate solution. The reaction was stopped with H2SO4 and the absorbance was measured at 490 nm. The results are shown as the arithmetic media ± standard deviation from three triplicate assays.