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Table 1 Features of the phlebotomine sand flies investigated in the experimental part of the study

From: Identification of phlebotomine sand flies using one MALDI-TOF MS reference database and two mass spectrometer systems

Species (morphological ID) Origin (field; colony) Collection year; storage until analyses in 2014 No. used for calculating reference database No. used in validation study
Lutzomyia (Lutzomyia) longipalpis 1 Brazil (colony3) 2014; 70 % EtOH for few days 5 5
Nyssomyia trapidoi 1 Ecuador (field) 2013; 96 % EtOH 5 5
Phlebotomus (Adlerius) arabicus 1 Israel (colony3) 2014; 70 % EtOH for few days 5 5
Phlebotomus (Euphlebotomus) argentipes 1 India (colony3) 2014; 70 % EtOH for few days 5 5
Phlebotomus (Larroussius) ariasi 1 Southern France (field) 2011; 70 % EtOH, −20 °C 5 7
Phlebotomus (Euphlebotomus) barguesae 1 Thailand (field) 2012; 96 % EtOH 5 6
Phlebotomus (Phlebotomus) duboscqi Senegal (colony) 2002; N2 then −80 °C4. 0 12
Phlebotomus (Phlebotomus) duboscqi 1 Senegal (colony3) 2014; 70 % EtOH for few days 5 5
Phlebotomus (Transphlebotomus) mascittii 1 France (colony) 2004; N2 then −80 °C4. 5 8
Phlebotomus (Larroussius) neglectus 1 Italy (field) 1991; N2 then −80 °C4. 5 2
Phlebotomus (Larroussius) orientalis 1 Ethiopia (colony3) 2014; 70 % EtOH for few days 5 5
Phlebotomus (Phlebotomus) papatasi Saudia Arabia (colony) 1994; N2 then −80 °C4. 0 7
Phlebotomus (Phlebotomus) papatasi 1 Turkey (colony3) 2014; 70 % EtOH for few days 5 5
Phlebotomus (Larroussius) perfiliewi transcaucasicus 1 Iran (field) 2010; 70 % EtOH 5 4
Phlebotomus (Larroussius) perniciosus Southern France (field) 2011; 70 % EtOH, −20 °C 0 6
Phlebotomus (Larroussius) perniciosus 2 Italy (field) 1991; N2 then −80 °C4. 0 11
Phlebotomus (Larroussius) perniciosus 2 Malta (field) 1989; N2 then −80 °C4. 0 12
Phlebotomus (Larroussius) perniciosus 1 Spain (colony3) 2014; 70 % EtOH for few days 5 5
Phlebotomus (Paraphlebotomus) sergenti Iran (field) 2010; 70 % EtOH   2
Phlebotomus (Paraphlebotomus) sergenti 1 Turkey, Israel (colony3) 2014; 70 % EtOH for few days 5 5
Phlebotomus (Larroussius) tobbi 2 Iran (field) 2010; 70 % EtOH 0 10
Phlebotomus (Larroussius) tobbi 1 Turkey (colony3) 2014; 70 % EtOH for few days 5 5
Phlebotomus (Larroussius) tobbi Greece (field) 1990; N2 then −80 °C4 0 2
Phlebotomus sp. Iran (field) 2010; 70 % EtOH 0 1
Psathyromyia (Foratiniella) aragaoi 1 Ecuador (field) 2013; 96 % EtOH 5 0
Psychodopygus panamensis 1 Ecuador (field) 2013; 96 % EtOH 5 2
Sergentomyia (Sergentomyia) dentata 1 Israel (field) 2000; N2 then −80 °C4. 5 7
Sergentomyia (Sergentomyia) minuta 1 Southern France (field) 2011; 70 % EtOH, −20 °C 5 7
Sergentomyia (Sergentomyia) schwetzi 1 Ethiopia (colony3) 2014; 70 % EtOH for few days 5 5
   Total 100 161
  1. 1Used for calculating reference database
  2. 2Used for calculating alternative reference database (replacing spectra from colony insects, see also Fig. 2)
  3. 3Colonies maintained at Department of Parasitology, Charles University, Prague, Czech Republic [15]
  4. 4-80 °C since 2012