Table 1 Characteristic of microsatellite loci for T. sordida
From: Markers for the population genetics studies of Triatoma sordida (Hemiptera: Reduviidae)
Locus | Primer sequences (5′- 3′) | T (°C) | MgCl2 50 mM(%) | bp | N |
|---|---|---|---|---|---|
Tb 830 | F: GTCAGATGCATGGTGATAC | 48 | 3 | 119-122(265–292) | 4(8) |
R: CATGGAAGATACCTAAACGG | |||||
Tb 8112 | F: GAATACGCCTATTCACAG | 54 | 3 | 78-80(78–96) | 3(6) |
R: GGATATGTATTTTAAGGGA | |||||
Tb 8124 | F: GCCACTGTGTTCTCATTCC | 59.5 | 3 | 209-248(209–253) | 12(10) |
R: TGGTGTGATGCTCAGAAGG | |||||
TDMS3 | F: TCAGATGACGAGGTGGATTG | 63 | 3 | 137-143(129–146) | 3(7) |
R: ACGACCTCAACATCCCTTTC | |||||
TDMS4 | F: CAGTTGTTCATCAGGAAGTGAATC | 54 | 6 | 160(150–186) | 1(20) |
R: GCTCAGAAAATATGTTCCCAGT | |||||
Tinfest_ms23 | F: CTCTTGCTGGTTGTGCACTG | 64 | 6 | 156-173(148–177) | 10(5) |
R: GTAAACGCCATCCTCACACC | |||||
Tinfest_ms42 | F: GACGCTCCAGCTATCGATTC | 66 | 6 | 205-224(206–246) | 9(15) |
R: GGCCAATTGGTTTGGTAG TG | |||||
Tp59 | F: ACTTAGGTGGGTATGGA | 53 | 3 | 121-149(120–128) | 9(4) |
R: CAGAGTAGTAGCGTATTGA | |||||
Tp20 | F: ACTGACTCCGAGAAAGTG | 57 | 3 | 125-144(170–206) | 7(15) |
R: TTCCTAAATCCAAACCCT | |||||
Tp544 | F: TGTTAGAATGAATGCCACTA | 55 | 3 | 142-213(148–172) | 7(8) |
R: GCAATACAATAGAGGACTGA |