Fig. 1

Sialostatin L2 inhibits the MIP-α and IP-10 production by dendritic cells in response to Borrelia burgdorferi. Dendritic cells were exposed to B. burgdorferi at MOI = 10 in the presence or absence of sialostatin L (3 μM) or sialostatin L2 (3 μM). After 24 h, supernatants were collected and used in proteome chemokine array. The chemokines were visualized by enhanced chemiluminescence and their amount is expressed as arbitrary units (a). Similarly, supernatants from cells treated as described above were analysed in ELISA assay for the presence of MIP-1α (b) and IP-10 (c). Graphs (in b, c) show results of one representative experiment out of at least three independent experiments performed