Fig. 3

Effect of sialostatin L2 and sialostatin L on the Erk1/2 signalling pathway activated by Borrelia burgdorferi in dendritic cells. Dendritic cells were seeded in 24-well plate. Next day DCs were incubated 2 h with sialostatin L2 and sialostatin L (both 3 μM) prior to the addition of Borreliae (MOI = 10) and further incubated for indicated times. Afterwards, cells were lysed and obtained protein extract was analysed by western blotting using antibodies against phosphorylated form of Erk1/2 and total Erk1/2 (a). Proteins were visualized by enhanced chemiluminescence. Relative phosphorylation/activity of Erk1/2 (b) kinase is shown; signal corresponding to phosphorylated kinase was adjusted to total kinase level. Three independent experiments were performed with sialostatin L2 and two with sialostatin L and representative blots are shown. Graph represent the average ± SD from 2 experiments, the phosphorylation of kinase achieved at 30 min upon Borrelia addition was set up to 1 to allow pooling of data