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Table 1 Sensitivity estimates for three M. ozzardi blood parasite detection assays

From: A field trial of a PCR-based Mansonella ozzardi diagnosis assay detects high-levels of submicroscopic M. ozzardi infections in both venous blood samples and FTA® card dried blood spots

Parasitism reference standard

Venous blood ModPCR assay

Dried blood spot MoPCR assay

Light microscopy assay

Samples testing positive with the venous blood ModPCR assay

 

27/47 (57.4 %) [105]

21/47 (44.7 %) [105]

44/96 (45.8 %) [214]

Samples testing positive with dried blood spot MoPCR assay

27/31 (87.1 %) [105]

 

18/31 (58.1 %) [105]

Samples testing positive with the light microscopy assay

21/24 (87.5 %) [105]

18/24 (75 %) [105]

 

44/52 (84.6 %) [214]

Samples testing positives with any other assay

31/37 (83.8 %) [105]

28/50 (56 %) [105]

24/52 (46.2 %) [105]

54/65 (83.1 %) [214]

47/101 (46.5 %) [214]

Sample testing positives with both other assays

17/18 (94.4 %) [105]

17/21 (81 %) [105]

17/27 (63 %) [105]

Samples testing positive with all three assays

47/53 (88.7 %) [105]

31/53 (58.5 %) [105]

24/53 (45.3 %) [105]

96/107 (89.7 %) [214]

 

52/107 (48.6 %) [214]

Assay sensitivity-estimate ranges

83.1 %–94.4 %

56 %–81 %

44.7 %–63 %

  1. Table 1 shows sensitivity estimates for three M. ozzardi blood parasite detection assays. Sensitivity calculations have been made using six different blood parasitism reference standards (as indicated). The number of blood samples which had test results included for the sensitivity estimates is given in square brackets. Estimates are divided into: (i) those based solely on data obtained from individuals from Tefé (for which data was obtained using all three assays) and for which 105 blood samples were assessed and (ii) the total datasets, which combines the data from Codajás with the Tefé data (for which there was a total 214 blood samples assessed)