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Table 1 Sensitivity estimates for three M. ozzardi blood parasite detection assays

From: A field trial of a PCR-based Mansonella ozzardi diagnosis assay detects high-levels of submicroscopic M. ozzardi infections in both venous blood samples and FTA® card dried blood spots

Parasitism reference standard Venous blood ModPCR assay Dried blood spot MoPCR assay Light microscopy assay
Samples testing positive with the venous blood ModPCR assay   27/47 (57.4 %) [105] 21/47 (44.7 %) [105]
44/96 (45.8 %) [214]
Samples testing positive with dried blood spot MoPCR assay 27/31 (87.1 %) [105]   18/31 (58.1 %) [105]
Samples testing positive with the light microscopy assay 21/24 (87.5 %) [105] 18/24 (75 %) [105]  
44/52 (84.6 %) [214]
Samples testing positives with any other assay 31/37 (83.8 %) [105] 28/50 (56 %) [105] 24/52 (46.2 %) [105]
54/65 (83.1 %) [214] 47/101 (46.5 %) [214]
Sample testing positives with both other assays 17/18 (94.4 %) [105] 17/21 (81 %) [105] 17/27 (63 %) [105]
Samples testing positive with all three assays 47/53 (88.7 %) [105] 31/53 (58.5 %) [105] 24/53 (45.3 %) [105]
96/107 (89.7 %) [214]   52/107 (48.6 %) [214]
Assay sensitivity-estimate ranges 83.1 %–94.4 % 56 %–81 % 44.7 %–63 %
  1. Table 1 shows sensitivity estimates for three M. ozzardi blood parasite detection assays. Sensitivity calculations have been made using six different blood parasitism reference standards (as indicated). The number of blood samples which had test results included for the sensitivity estimates is given in square brackets. Estimates are divided into: (i) those based solely on data obtained from individuals from Tefé (for which data was obtained using all three assays) and for which 105 blood samples were assessed and (ii) the total datasets, which combines the data from Codajás with the Tefé data (for which there was a total 214 blood samples assessed)