Skip to main content

Table 2 M. ozzardi prevalence estimates for the river Rio Solimões region

From: A field trial of a PCR-based Mansonella ozzardi diagnosis assay detects high-levels of submicroscopic M. ozzardi infections in both venous blood samples and FTA® card dried blood spots

  Codajás Tefé Rio Solimões combined data
Venous blood ModPCR prevalence estimates 49/109 (45 %) 47/105 (44.8 %) 96/214 (44.9 %)
Dried blood spot ModPCR prevalence estimates   31/105 (29.5 %)  
Light microscopy prevalence estimates 25.7 % (28/109) 22.9 % (24/105) 24.3 % (52/214)
Sub-microscopic infection prevalence estimates VB-ModPCR: 20.2 % (22/109) VB-ModPCR: 24.8 %(26/105) VB-ModPCR: 22.4 % (48/214)
DBS-ModPCR: 11.4 %(12/105) DBS or VB ModPCR: 23.8 % (51/214)
DBS or VB ModPCR: 27.6 %(29/105)
Combined data prevalence estimates VB-ModPCR & LM: 45.9 % (50/109) VB-ModPCR: 47.6 % (50/105) VB-ModPCR: 46.7 % (100/214)
DBS-ModPCR: 34.3 % (36/105) DBS or VB ModPCR: 48.6 % (104/214)
DBS or VB ModPCR: 51.4 % (54/105)
Light microscopy false negatives VB-ModPCR & LM: ≥27.2 % (≥22/81) VB-ModPCR: ≥32.1 % (≥26/81) VB-ModPCR: 2.08
DBS-ModPCR: ≥14.8 % (≥12/81) DBS-ModPCR: 1.5
DBS or VB ModPCR:≥35.8 % (≥29/81) DBS or VB ModPCR: 2.25
Light microscopy prevalence underestimating VB-ModPCR & LM: 1.79 × VB-ModPCR: 2.08 × VB-ModPCR:1.92 ×
DBS-ModPCR: 1.5 × DBS or VB ModPCR: 2 ×
DBS or VB ModPCR: 2.25 ×
  1. Table 2 shows M. ozzardi summary prevalence estimates for the Rio Solimões as a whole and divided by the two study areas. Estimates are also shown divided into infections that were detected (patent) in light microscopy and infections that were not detected by light microscopy, but were detected by PCR: sub-microscopic infections. Additionally, the table shows a breakdown of sub-microscopic parasite detection and prevalence estimates made by different assaying methods. Corresponding calculations based on each assay's submicroscopic parasite detection have also been used to estimate the minimum number of light microscopy (LM) false negatives and prevalence underestimating for M. ozzardi parasite infections. All calculations neglect the possibility of false positives. Dried blood spot is abbreviated to DBS and venous blood to VB