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Fig. 5 | Parasites & Vectors

Fig. 5

From: Expression of active trypsin-like serine peptidases in the midgut of sugar-feeding female Anopheles aquasalis

Fig. 5

Detection of proteolytic activity from the midgut of An. aquasalis females using the fluorogenic substrate Z-Phe-Arg-AMC. a The proteolytic activity was detected using the substrate in the absence (control) or presence of 1 mM PMSF, 100 μM TLCK, 100 μM TPCK, 10 μM E-64 in 100 mM Tris–HCl buffer, pH 7.5. b Effect of pH on the proteolytic activity. Assays were performed at 37 °C in a 100 mM sodium acetate (pH 3.5 and 5.5) buffer or 100 mM Tris–HCl buffer (pH 7.5 and 10)

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