Fig. 5

Example of FISH for supercontig 3.3. RGB is a view with Red, Green and Blue channels merged; R – Red channel; G – Green channel, B – Blue channel. The red signal corresponds to the gene CPIJ000183, and the blue signal to CPIJ000244. The results of FISH together with knowledge of the base pair location of each exon within genomic supercontig (see Table 1), allowed inferring directionality of the supercontig within the chromosome as “forward” (see Fig. 3 to find supercontig 3.3 on the map). Refer to Fig. 2 to distinguish telomeres, as the same nucleus was used in both Fig. 2 and Fig. 5