Saliva from nymph and adult females of Haemaphysalis longicornis: a proteomic study

Tirloni, Lucas; Islam, Mohammad Saiful; Kim, Tae Kwon; Diedrich, Jolene K.; Yates, John R.; Pinto, Antônio F. M.; Mulenga, Albert; You, Myung-Jo; Da Silva Vaz, Itabajara

doi:10.1186/s13071-015-0918-y

Parasites & Vectors

Table 2 Tick and host proteins identified exclusively in adult saliva

From: Saliva from nymph and adult females of Haemaphysalis longicornis: a proteomic study

Contig number^a	Annotation	Class	Spec count
TICK
JAA54320	glycine-rich protein	Glycine-rich protein	1.50
EEC14126	radixin/ezrin/moesin	Cytoskeletal	2.00
ACX53929	putative beta thymosin	Cytoskeletal	1.33
DAA34555	microtubule-binding protein	Cytoskeletal	1.00
AEO32824	actin depolymerizing factor	Cytoskeletal	1.50
AEO33976	beta tubulin	Cytoskeletal	6.50
AFR32950	paramyosin	Cytoskeletal	5.33
JAB76162	dynein light chain	Cytoskeletal	1.00
JAB80373	myosin class i heavy chain	Cytoskeletal	12.25
AAY42205	troponin T	Cytoskeletal	1.00
Rm-1533	beta tubulin partial	Cytoskeletal	6.33
Rm-80704	myosin class ii heavy chain	Cytoskeletal	4.67
ACF35539	glutathione S-transferase	Oxidant metabolism/detoxification	6.50
ACG76272	aldehyde dehydrogenase	Oxidant metabolism/detoxification	1.00
AAQ74441	glutathione S-transferase	Oxidant metabolism/detoxification	1.50
AEO33057	sulfotransferase	Oxidant metabolism/detoxification	2.00
AEO35358	catalase	Oxidant metabolism/detoxification	8.00
JAA63098	putative aldehyde dehydrogenase	Oxidant metabolism/detoxification	3.00
Rm-14504	aldehyde dehydrogenase	Oxidant metabolism/detoxification	14.75
Rm-46289	aldehyde dehydrogenase	Oxidant metabolism/detoxification	2.00
ADN34303	cuticular protein	Extracellular matrix/cell adhesion	4.75
AEO34631	phosphoserine aminotransferase	Metabolism, amino acid	1.50
Rm-73466	homocysteine S-methyltransferase	Metabolism, amino acid	2.50
AEO32930	transketolase	Metabolism, carbohydrate	2.33
ACH88101	glyceraldehyde-3-phosphate dehydrogenase	Metabolism, energy	2.67
AEO32901	ATP synthase subunit beta	Metabolism, energy	7.75
AEO34579	3-phosphoglycerate dehydrogenase	Metabolism, energy	3.75
AEO35473	Isocitrate dehydrogenase NADP	Metabolism, energy	6.00
JAA60091	3-phosphoglycerate dehydrogenase	Metabolism, energy	3.33
JAA60302	Isocitrate dehydrogenase NADP	Metabolism, energy	7.00
JAA62224	imp cyclohydrolase/methylglyoxal synthase	Metabolism, energy	8.75
JAA66712	C-1-tetrahydrofolate synthase	Metabolism, energy	2.00
JAA68969	glyceraldehyde-3-phosphate dehydrogenase	Metabolism, energy	2.50
JAB74970	cytochrome B5	Metabolism, energy	1.33
Rm-26196	Malate dehydrogenase	Metabolism, energy	2.67
Rm-18758	15-hydroxyprostaglandin dehydrogenase	Metabolism, lipid	1.50
Rm-73635	farnesoic acid o-methyltransferase	Metabolism, lipid	1.67
JAA59537	3’(2’)5’-bisphosphate nucleotidase	Metabolism, nucleotide	1.00
JAA60240	GDP dissociation inhibitor	Metabolism, nucleotide	1.50
	Inosine-5’-monophosphate dehydrogenase	Metabolism, nucleotide	4.75
Rm-32846	purine nucleoside phosphorylase transferase	Metabolism, nucleotide	2.00
Rm-6587	SAICAR synthase	Metabolism, nucleotide	2.00
AEO35504	annexin	Protein export machinery	1.00
BAF63673	protein disulfide-isomerase	Protein modification machinery	2.50
EEC14106	multifunctional chaperone	Protein modification machinery	2.50
JAA60036	heat shock protein	Protein modification machinery	1.50
JAA67522	heat shock protein hsp 90-alpha isoform	Protein modification machinery	2.50
JAB73342	methyltransferase	Protein modification machinery	1.50
JAB75807	cysteine s-methyltransferase	Protein modification machinery	2.00
Rm-24571	heat shock protein 70 cognate	Protein modification machinery	6.50
Rm-62470	heat shock-related protein	Protein modification machinery	3.75
JAA62377	gmp synthase	Protein modification machinery	2.50
AEO32591	elongation factor 1-alpha	Protein synthesis machinery	8.33
AEO32633	AAA+ ATPase	Protein synthesis machinery	1.00
Rm-50382	elongation factor 2-like	Protein synthesis machinery	4.00
AEO34773	secreted protein	Secreted conserved protein	3.00
AEO32884	lipocalin	Lipocalin	7.25
Rm-26539	lipocalin	Lipocalin	5.00
BAF51711	tick legumain	Proteinase	8.50
BAH86062	cathepsin L-like	Proteinase	2.33
JAA60171	metalloexopeptidase	Proteinase	3.00
JAA65860	CNDP dipeptidase	Proteinase	2.33
EEC20000	alpha-2-macroglobulin	Proteinase inhibitor	6.00
BAH02683	haemalin	Proteinase inhibitor	4.50
AEO35673	TIL	Proteinase inhibitor	5.00
BAG41813	cyclophilin 1	Signal transduction	3.50
AEO32842	regulatory protein mlp	Signal transduction	2.00
JAB75372	calmodulin	Signal transduction	1.67
Rm-69447	neuropeptide-like protein 31	Signal transduction	1.00
Rm-79606	Regucalcin	Signal transduction	2.50
Rm-154439	hemelipoprotein	Heme/iron metabolism	1.67
JAA62029	zinc finger protein	Transcription machinery	1.00
EEC05877	apolipophorin	Transporters/receptors	2.75
JAB78095	glutamate receptor 1-like	Transporters/receptors	1.67
RABBIT
G1U9R8	gelsolin	Cytoskeletal	2.50
P27170	serum paraoxonase/arylesterase 1	Oxidant metabolism/detoxification	1.00
G1SR65	myeloperoxidase	Oxidant metabolism/detoxification	1.50
G1T049	collagen alpha-6(IV) chain	Extracellular matrix/cell adhesion	1.00
G1TZA1	type X collagen alpha 1-like	Extracellular matrix/cell adhesion	2.25
O77791	protein S100-A12	Immunity	4.50
P01840	Ig kappa-b4 chain C region	Immunity	10.25
P01870	Ig gamma-1 chain C region	Immunity	48.50
P04221	Ig mu chain C region	Immunity	4.00
G1SS91	complement C4-A	Immunity	3.75
G1T3X1	complement component C9	Immunity	2.00
G1TEC1	Ig kappa chain V-I	Immunity	4.75
G1TFU1	Ig kappa chain V-I	Immunity	5.25
G1TKP3	immunoglobulin lambda-like	Immunity	4.00
G1TM51	Ig kappa chain V-I	Immunity	4.00
G1TPF2	T-cell surface glycoprotein CD8 -like	Immunity	5.00
G1TPZ1	galectin-1	Immunity	2.33
G1TRW8	neutrophil gelatinase-associated lipocalin	Immunity	8.50
G1TUX5	C1q and TNF related protein 7-like	Immunity	2.50
G1TVN7	Ig kappa chain V-I	Immunity	5.75
G1TVZ5	T-cell surface glycoprotein CD8 -like	Immunity	1.50
Q9GK63	mammaglobin-B	Immunity	2.00
Q9GK67	secretoglobin	Immunity	3.67
U3KM01	Ig kappa chain V-I	Immunity	4.00
G1SQA8	ATP synthase mitochondrial precursor	Metabolism, energy	3.50
G1TS29	triosephosphate isomerase	Metabolism, energy	1.33
G1TYA7	L-lactate dehydrogenase B chain	Metabolism, energy	2.67
G1TB24	peroxysomal Fatty Acyl CoA Transporter	Metabolism, lipid	1.50
G1SN21	purine nucleoside phosphorylase	Metabolism, nucleotide	2.00
G1TXI1	nucleophosmin	Metabolism, nucleotide	3.33
G1U9T4	nucleoside diphosphate kinase B	Metabolism, nucleotide	3.33
G1U155	histone H2B type 1-like	Nuclear regulation	3.00
P51662	annexin A1	Protein export machinery	5.50
G1TDI4	transthyretin	Protein export machinery	3.00
G1SJZ9	thioredoxin	Protein modification machinery	1.33
G1SEK8	metalloendopeptidase inhibitor	Proteinase inhibitor	6.75
G1SIK0	antithrombin III	Proteinase inhibitor	8.67
G1SZA4	inter-alpha-trypsin inhibitor heavy chain H1	Proteinase inhibitor	2.00
G1TM88	alpha-1-antitrypsin	Proteinase inhibitor	2.33
G1U6R8	negative regulation of endopeptidase	Proteinase inhibitor	8.25
Q45GR2	alpha-2-antiplasmin	Proteinase inhibitor	3.50
G1SNP6	serine/threonine-protein kinase	Signal transduction	1.50
P09809	apolipoprotein A-I	Heme/iron metabolism	4.25
G1TFW8	lactotransferrin	Heme/iron metabolism	5.75
G1SU82	vitamin D-binding protein	Transporters/receptors	4.75
G1T4K1	sodium channel protein type 3 isoform X1	Transporters/receptors	2.00
G1TP66	keratin type II cuticular Hb3	Keratin	1.50
P00919	carbonic anhydrase 2	Hemoglobin/RBC products	3.50

^aProtein and spectral count of host and tick proteins identified in adult Haemaphysalis longicornis saliva. Annotation and accession numbers of best match identities obtained using BLASTP against the several protein databases can be checked with more details in Additional file 1: Table S1 and Additional file 2: Table S2

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ISSN: 1756-3305

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