Table 1 Pathogens and their corresponding probe sequences used to detect pathogen DNA
From: Infection and exposure to vector-borne pathogens in rural dogs and their ticks, Uganda
Region amplified | Foward primer (5′-3′) | Reverse primer (5′-3′) | Reference | Final [primer] (μM) | |
|---|---|---|---|---|---|
Anaplasmataceae | 16S rRNA | GCAAGCYTAACACATGCAAGTCG | CTACTAGGTAGATTCCTAYGCATTACTCACC | In house | 0.5 |
Piroplasmida | 18S rRNA | GACGATCAGATACCGTCGTAGTCC | CAGAACCCAAAGACTTTGATTTCTCTC | In house | 0.3 |
Rickettsia sp. | ITS2 | GCTCGATTGRTTTACTTTGCTGTGAG | CATGCTATAACCACCAAGCTAGCAATAC | In house | 0.5/0.3 |
Bartonella sp. | ITS1 | AGATGATGATCCCAAGCCTTCTG | CCTCCGACCTCACGCTTATCA | 0.3 | |
Primers used for sequencing | |||||
Ehrlichia sp. | GGAATCTACCTAGTAGTACGGAATAGCYA | GTAGGTACCGTCATTATCTTCCCYAY | In house | ||
Rickettsia sp. | RACGGACTAATTRGRGCT | CATTATCTTCCYTGCTAAAAGAG | In house | ||