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Fig. 2 | Parasites & Vectors

Fig. 2

From: Development of a multiplex fluorescence immunological assay for the simultaneous detection of antibodies against Cooperia oncophora, Dictyocaulus viviparus and Fasciola hepatica in cattle

Fig. 2

Results for the triplex assay using serum dilutions of cattle infected with Dictyocaulus, Fasciola and Cooperia. Five parameter logistic regression curves were calculated based on median fluorescence intensity (MFI) values. Beads are coupled with recombinant antigen for the detection of Cooperia oncophora (green), Dictyocaulus viviparus (black) and Fasciola hepatica (red). Artificial mixtures of sera from animals infected with the target species was used. Dilutions are presented as 0.005 1:200

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