Fig. 1From: Serological and molecular diagnostic surveys combined with examining hematological profiles suggests increased levels of infection and hematological response of cattle to babesiosis infections compared to native buffaloes in EgyptAgarose gel electrophoresis analysis: Serial dilutions of known amounts of purified plasmid DNA amplified by PCR. Molecular size ladder in base pair (bp), 100Â bp DNA ladder, (lane M). a. Plasmid pCRâ„¢ 2.1-TOPO-B. bovis rra amplified using snPCR. The amounts of plasmid amplified are as follows: 10 femtogram (fg) (lane 1), 1Â fg (lane 2), 0.1Â fg (lane 3), 0.01Â fg (lane 4), 0.001Â fg (lane 5), negative control, no plasmid (lane 6). b. Plasmid pCRâ„¢ 2.1-TOPO-B. bigemina rap-1c amplified using nPCR. The amounts of plasmid amplified are as follows: 10Â fg (lane 1), 1Â fg (lane 2), 0.1Â fg (lane 3), 0.01Â fg (lane 4), 0.001Â fg (lane 5), negative control, no plasmid (lane 6)Back to article page