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Fig. 1 | Parasites & Vectors

Fig. 1

From: Serological and molecular diagnostic surveys combined with examining hematological profiles suggests increased levels of infection and hematological response of cattle to babesiosis infections compared to native buffaloes in Egypt

Fig. 1

Agarose gel electrophoresis analysis: Serial dilutions of known amounts of purified plasmid DNA amplified by PCR. Molecular size ladder in base pair (bp), 100 bp DNA ladder, (lane M). a. Plasmid pCR™ 2.1-TOPO-B. bovis rra amplified using snPCR. The amounts of plasmid amplified are as follows: 10 femtogram (fg) (lane 1), 1 fg (lane 2), 0.1 fg (lane 3), 0.01 fg (lane 4), 0.001 fg (lane 5), negative control, no plasmid (lane 6). b. Plasmid pCR™ 2.1-TOPO-B. bigemina rap-1c amplified using nPCR. The amounts of plasmid amplified are as follows: 10 fg (lane 1), 1 fg (lane 2), 0.1 fg (lane 3), 0.01 fg (lane 4), 0.001 fg (lane 5), negative control, no plasmid (lane 6)

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