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Table 1 Primer targets, sequences and amplicon base pair size for previously unreported quantitative PCR assays used in this study. F: Forward primer; R: reverse primer

From: Serological and molecular analysis of feline vector-borne anaplasmosis and ehrlichiosis using species-specific peptides and PCR

Target Primers Amplicon (bp)
Anaplasma -tr-1 F- 5′ ATGTTTATGACTTCTCAAGCAC-3′ R- 5′ CCC TTT TCG TAT TTT TGT AC-3′ 200
A. phagocytophilum -p44 F- 5′ TGGTGGTGCGGGATATTTCTATGTTG-3′ R- 5′ CCCAATCCGAGGATCAGGTGTG-3′ 179
A. platys -p44 F- 5′ GCT AAG TGG AGC GGT GGC GAT GAC AG-3′ R- 5′ GCCGCAGTTTCCCCGGTACT-3′ 179
E. chaffeensis -nadA F- 5′ CGCAAAAGATGTAATTCTTTGGGAATC-3′ R- 5′ CACCTCAAAATCAGAATTCATCGAAGG-3′ 117
E. ewingii -sodB F- 5′ GCTGGAATAGGTCATTTTGGTAGTGGA-3′ R- 5′ GTTCCCATACATCCATAGCAAGCAAC-3′ 147